Fig. 1: P110δ might be a significant mediator in TiPs-induced macrophages inflammation.
From: MiR-92a/KLF4/p110δ regulates titanium particles-induced macrophages inflammation and osteolysis
A Representative image of Hematoxylin-Eosin (H&E) staining and immunohistochemical (IHC) staining for TNFα and IL-6 of human synovial membrane from femoral head necrosis (FHN) patients and aseptic loosening (AL) patients. The black bar, 50 μm. B Average intensity score (IS) of IHC staining was calculated by Bresalier’s analysis. C, D The mRNA expressions (C) and serum levels (D) of TNFα and IL-6 in RAW264.7 were ascertained by qPCR and ELISA assay upon different stimulus for various periods. E The Signaling Pathways Project (SPP) database indicated that the expression of p110δ was elevated upon various pro-inflammatory stimulation including TNFα, IL-1β, interferon γ, LPS, etc. F Protein–protein interaction network (PPIN) from STRING database depicted a strong connection between p110δ and pro-inflammatory cytokines. All data were concluded from at least three independent assays. Statistic data were displayed as mean ± SEM and were conducted unpaired t test analysis or one-way ANOVA analysis to determine significant difference. *p < 0.05, **p < 0.01, ***p < 0.001 compared with the negative group.
