Fig. 4: Pik3cd transcription was mediated by KLF4 in TiPs-stimulated macrophages.
From: MiR-92a/KLF4/p110δ regulates titanium particles-induced macrophages inflammation and osteolysis
A Predicted sequence logo of KLF4 binding site was acquired by JASPAR database. B mRNA expression of p110δ from KLF4-knockdown RAW264.7 or KLF4-overexpressed RAW264.7 was detected. C Protein level of p110 from KLF4-knockdown RAW264.7 or KLF4-overexpressed RAW264.7 was detected. D Structure of plasmids containing mutant-type of Pik3cd promoter for luciferase reporter assay. E Relative luciferase activity upon different supplement of KLF4-overexpressed (KLF4+) plasmids. F Relative luciferase activity was analysed after transfecting plasmids containing wild-type (Pik3cd-WT) or mutant-type (Pik3cd-MT) of Pik3cd promoter with or without KLF4+ plasmids. All data were concluded from at least three independent assays. Statistic data were displayed as mean ± SEM and were conducted unpaired t test analysis or one-way ANOVA analysis to determine significant difference. *p < 0.05, **p < 0.01, ***p < 0.001 compared with the negative group.
