Table 1 Primers for cDNA synthesis or qPCR detection of RT-qPCR.
From: MiR-92a/KLF4/p110δ regulates titanium particles-induced macrophages inflammation and osteolysis
Primer names | Sequences |
|---|---|
TNFα | F: CAGGCGGTGCCTATGTCTC |
R: CGATCACCCCGAAGTTCAGTAG | |
IL-6 | F: GAAATGCCACCTTTTGACAGTG |
R: TGGATGCTCTCATCAGGACAG | |
p110δ | F: GTAAACGACTTCCGCACTAAGA |
R: GCTGACACGCAATAAGCCG | |
KLF4 | F: AGGAACTCTCTCACATGAAGCG |
R: GGTCGTTGAACTCCTCGGTC | |
GAPDH | F: TGTGTCCGTCGTGGATCTGA |
R: TTGCTGTTGAAGTCGCAGGAG | |
mmu-miR-32-5p | F: GCGGGCTATTGCACATTACTA |
R: GGTCGTTGAACTCCTCGGTC | |
RT: GTCGTATCCAGTGCAGGGTCCGAG | |
GTATTCGCACTGGATACGACTGCAACT | |
mmu-miR-148b-3p | F: GCGGGCTCAGTGCATCACAGA |
R: GCAGGGTCCGAGGTATTC | |
RT: GTCGTATCCAGTGCAGGGTCCGAG | |
GTATTCGCACTGGATACGACACAAAGT | |
mmu-miR-92a-3p | F: GCGGGCTATTGCACTTGTCCC |
R: GCAGGGTCCGAGGTATTC | |
RT: GTCGTATCCAGTGCAGGGTCCGAG | |
GTATTCGCACTGGATACGACCAGGCCG | |
mmu-miR-92b-3p | F: GCGGGCTATTGCACTCGTCCC |
R: GCAGGGTCCGAGGTATTC | |
RT: GTCGTATCCAGTGCAGGGTCCGAG | |
GTATTCGCACTGGATACGACGGAGGCC | |
mmu-miR-152-3p | F: GCGGGCTCAGTGCATGACAGA |
R: GCAGGGTCCGAGGTATTC | |
RT: GTCGTATCCAGTGCAGGGTCCGAG | |
GTATTCGCACTGGATACGACCCAAGTT | |
U6 | F: TGCGGGTGCTCGCTTCGGCAGC |
R: CCAGTGCAGGGTCCGAGGT | |
RT: GTCGTATCCAGTGCAGGGTCCGAGG | |
TGCACTGGATACGACAAAATATGGAAC |
