Fig. 2: Depletion of GBP2 impairs proliferation and migration in glioma cells.
From: GBP2 facilitates the progression of glioma via regulation of KIF22/EGFR signaling
A GBP2 was depleted efficiently. Western blotting results for GBP2 knockdown in U87 and U251 cells. B CCK8 assay results for cell proliferation in U87 and U251 cells transfected with siCtrl, siGBP2#1, or siGBP2#2. C Colony formation assay results of U87 and U251 cells transfected with siCtrl, siGBP2#1, or siGBP2#2. Clusters of more than 50 cells were identified as colonies. D Apoptosis results for U87 and U251 cells transfected with siCtrl, siGBP2#1, or siGBP2#2 using annexin V and PI. E Cell cycle results for U87 and U251 cells transfected with siCtrl, siGBP2#1, or siGBP2#2 using PI staining. F Transwell assay results for U87 and U251 cells transfected with siCtrl, siGBP2#1, or siGBP2#2. Each experiment repeated as least 3 times. Magnification: ×10. *P < 0.05, **P < 0.01, ***P < 0.001.
