Fig. 1: Effect of a high-fat diet on hepatic HtrA2/Omi expression, limiting enzyme of β-oxidation, and autophagy-associated proteins in the liver during NAFLD progression.

A Western blot analysis of HtrA2/Omi, CPT1α, PPARα, p62, and LC3I/II in liver tissues. The histogram shows the ratio quantification of band intensities (n = 3). B Immunohistochemical staining of CPT1α, PPARα, and LC3II in the liver. scale bars: 20 μm (n = 6). Data are shown as means ± SEM. Student’s t test was used. **P < 0.01 vs. control group.