Fig. 2: Expression of HtrA2/Omi improves metabolic parameters and decreases hepatic steatosis in HFD-fed obese mice. | Cell Death Discovery

Fig. 2: Expression of HtrA2/Omi improves metabolic parameters and decreases hepatic steatosis in HFD-fed obese mice.

From: HtrA2/Omi mitigates NAFLD in high-fat-fed mice by ameliorating mitochondrial dysfunction and restoring autophagic flux

Fig. 2

A Experiment design and time course. Groups were established as follows: Control mice (n = 7), HFD + AAV8-TBG-mNeongreen mice (n = 7) and HFD + AAV8-TBG-mNeongreen-HtrA2/Omi mice (n = 7). B The mNeongreen expression of liver tissues was observed under a fluorescent microscope, scale bars: 50 μm (up), 20 μm (down) (n = 6). C Western blot analysis of HtrA2/Omi in liver homogenates from the three groups (n = 3). D Bodyweight was monitored from W1 to W14 (n = 7). E Food intake from W1 to W14 (n = 7). F H&E staining of liver histology (up) and Oil Red O staining of liver lipid accumulation (down) at W14, scale bars: 50 μm (n = 7). G Comparisons of ALT and AST serum levels in treatment groups (n = 7). H The serum levels of lipid in different groups (n = 7). I Analyses of hepatic TG levels in mice (n = 7). J Measurement of plasma glucose during GTT (left); the AUC for GTT was calculated (right) (n = 7). K Blood-glucose levels (left) and the area under the curve (right) for glucose during ITT (n = 7). Data are shown as means ± SEM. One-way ANOVA was used. *P < 0.05 vs. control group; **P < 0.01 vs. control group; #P < 0.05 vs. HFD + AAV8-TBG-mNeongreen group; ##P < 0.01 vs. HFD + AAV8-TBG-mNeongreen group.

Back to article page