Fig. 3: Hepatic HtrA2/Omi expression protects against mitochondrial impairment and enhances autophagy activity in fatty liver. | Cell Death Discovery

Fig. 3: Hepatic HtrA2/Omi expression protects against mitochondrial impairment and enhances autophagy activity in fatty liver.

From: HtrA2/Omi mitigates NAFLD in high-fat-fed mice by ameliorating mitochondrial dysfunction and restoring autophagic flux

Fig. 3

A Representative TEM images of hepatic mitochondria. Mitochondrial morphology and integrity were observed. Scale bar (up): 0.5 μm; scale bar (down): 2 μm (n = 7). B Hepatic mtDNA content in different groups (n = 7). C The levels of ATP in the liver (n = 7). D The expression of genes involved in lipid metabolism in the livers (n = 7). E Western blot analysis of CPT1α, PPARα, p62, and LC3I/II in liver tissues. The histogram shows the ratio quantification of band intensities (n = 3). F Images from TEM show characteristic autophagosomes (red arrows) in hepatocytes. scale bar: 1 μm (n = 7). G Immunohistochemical staining of CPT1α, PPARα, and LC3II in the liver, scale bars: 20 μm (n = 6); immunofluorescence of LC3 puncta, scale bars: 10 μm (n = 6). Data are shown as means ± SEM. One-way ANOVA was used. **P < 0.01 vs. control group; #P < 0.05 vs. HFD + AAV8-TBG-mNeongreen group; ##P < 0.01 vs. HFD + AAV8-TBG-mNeongreen group.

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