Fig. 4: Indirect calorimetry analysis showing hepatic HtrA2/Omi enhances respiratory exchange ratio (RER) and heat production in NAFLD mice.

A The CO2 production (VCO2) during 12-h dark/12-h light cycles measured in a metabolic cage (left) and the average VCO2 values were calculated (right) (n = 6). B The oxygen consumption (VO2) during two 12-h dark/12-h light cycles measured in a metabolic cage (left) and the average VO2 values were calculated (n = 6). C The RER measured in a metabolic cage (left) and the average RER (right) was calculated (n = 6). D The heat production measured in a metabolic cage (left) and the average values (right) was calculated (n = 6). Data are shown as means ± SEM. One-way ANOVA was used. *P < 0.05 vs. control group; **P < 0.01 vs. control group; ##P < 0.01 vs. HFD + AAV8-TBG-mNeongreen group.