Fig. 5: Effect of FFA on HtrA2/Omi expression, limiting enzyme of β-oxidation, and autophagy-associated proteins in vitro model of NAFLD.

A Nile red staining of cells, scale bars: 50 μm (n = 3). B Western blot analysis of HtrA2/Omi, CPT1α, PPARα, p62, and LC3I/II in different groups. The histogram shows the ratio quantification of band intensities (n = 3). Data are shown as means ± SEM. Student’s t test was used. **P < 0.01 vs. control group.