Fig. 3: Lorlatinib-induced autophagy protects cells from apoptosis. | Cell Death Discovery

Fig. 3: Lorlatinib-induced autophagy protects cells from apoptosis.

From: Protective autophagy decreases lorlatinib cytotoxicity through Foxo3a-dependent inhibition of apoptosis in NSCLC

Fig. 3: Lorlatinib-induced autophagy protects cells from apoptosis.The alternative text for this image may have been generated using AI.

A Cells were treated with lorlatinib (10 nM) with or without rapamycin (500 nM) for 48 h and observed changes in the content of LC3, p62, p-mTOR and total mTOR. B Western blot analysis of cells treated with or without fetal bovine serum (FBS) for 48 h. C Fluorescence photographs were shown the autophagosomes of H3122 and H2228 cells, which were treated with Rap (500 nM) or serum deprivation. Scale bar is 40 μm. D Cell viability CCK-8 assay for cells treated with the indicated concentrations of lorlatinib with or without CQ/ rapamycin/FBS for 48 h. *P < 0.01 compared with control. E Cells were treated with lorlatinib with or without CQ for 48 h, then stained with AO/EB. *P < 0.01. F Cells were treated by lorlatinib with or without CQ for 48 h. Western blot analysis was performed on the Bim, BCL-2, MCL-1, P-MCL-1, BCL-XL expression levels. GAPDH was included as a loading control. Lor lorlatinib, CQ chloroquine, Rap rapamycin.

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