Fig. 1: First-line chemotherapy effectively inhibits GC growth but develops drug resistance. | Cell Death Discovery

Fig. 1: First-line chemotherapy effectively inhibits GC growth but develops drug resistance.

From: Targeting IL8 as a sequential therapy strategy to overcome chemotherapy resistance in advanced gastric cancer

Fig. 1

A Schematic representation of the BGC823-bearing model of first-line chemotherapy resistance. B The growth curves of tumor (n = 8 per group). C Hierarchical clustering gene transcription altered at different periods of chemotherapy. D, E The half-maximal inhibitory concentration (IC50) was measured by CCK8 assay. Primary cells from dissected tumor tissues were treated with different concentrations of 5-Fu (0, 5, 10, 20, 40, and 80 μM) and oxaliplatin (0, 2, 4, 8, 16 and 32 μM). F, G Flow cytometric assay of primary cells from dissected tumor tissues. Data are mean ± standard deviation. *P < 0.05. H Protein expression of apoptosis-related proteins (PARP, cleaved PARP, Bcl2, and Bax) were detected by western blot analysis in dissected tumor tissues. I The mRNA expression of Bax, Bcl2, and ki-67 in dissected tumor tissues were detected by qRT-PCR assay. Data are mean ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001.

Back to article page