Fig. 4: Sequential treatment with first-line, second-line chemotherapy, and reparixin preserves efficacy to GC cells and ameliorates toxicity to normal cells. | Cell Death Discovery

Fig. 4: Sequential treatment with first-line, second-line chemotherapy, and reparixin preserves efficacy to GC cells and ameliorates toxicity to normal cells.

From: Targeting IL8 as a sequential therapy strategy to overcome chemotherapy resistance in advanced gastric cancer

Fig. 4: Sequential treatment with first-line, second-line chemotherapy, and reparixin preserves efficacy to GC cells and ameliorates toxicity to normal cells.

A Schematic representation of sequential treatment with first-line, second-line chemotherapy, and reparixin in BGC823 or GES-1 cells. B, C Colony formation assays were performed on BGC823 and GES-1 cells treated with different drugs as in Fig. 4A. Data are mean ± standard deviation. *P < 0.05. D–F BGC823 and GES-1 cells were treated as in Fig. 4A and subjected to flow cytometric analysis. G–J Representative images (G, H) and quantification (I, J) of BGC823 and GES-1 cells treated as in Fig. 4A and then stained for γH2AX and DAPI. Data are mean ± standard deviation. ***P < 0.001. K–M BGC823 and GES-1 cells were treated as in Fig. 4A and subjected to Comet analysis. DNA damage is quantified as percent DNA in tails. Data are mean ± standard deviation. *P < 0.05, **P < 0.01.

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