Fig. 2: SMYD2 inhibition represses the proliferation and accelerates the senescence of GIST cells through reducing EZH2 expression.
From: SMYD2 aggravates gastrointestinal stromal tumor via upregulation of EZH2 and downregulation of TET1
A RT-qPCR and western blot analysis of EZH2 expression in GIST-T1 cells treated with LLY-507 (20 nM). GIST-T1 cells were treated with LLY-507 or combined with sh-EZH2. B Western blot analysis of EZH2 protein expression in GIST-T1 cells. C CCK-8 assay of GIST-T1 cell viability. D GIST-T1 cell colony formation ability evaluated by soft agar colony formation assay. E SA-β-gal detection of the proportion of senescent GIST-T1 cells. F GIST-T1 cell cycle distribution measured by BrdU/PI cell cycle assay. G Annexin V-FITC/PI double staining of GIST-T1 cell apoptosis. *p < 0.05 vs. control or sh-NC- or sh-NC + LLY-507-treated GIST-T1 cells. Measurement data were expressed as mean ± standard deviation. Unpaired t-test was used for two-group data comparison. The cell experiment was repeated three times.
