Fig. 2: FAM126A enhances proliferation, migration, and invasion in PC cells. | Cell Death Discovery

Fig. 2: FAM126A enhances proliferation, migration, and invasion in PC cells.

From: FAM126A interacted with ENO1 mediates proliferation and metastasis in pancreatic cancer via PI3K/AKT signaling pathway

Fig. 2: FAM126A enhances proliferation, migration, and invasion in PC cells.The alternative text for this image may have been generated using AI.

A FAM126A amounts in six PC cell lines and noncancerous pancreatic ductal epithelial (HPDE) cells. FAM126A mRNA and protein amounts were assessed by quantitative reverse transcriptase (qRT-PCR, upper panel) and immunoblot (lower panel) in AsPC-1, BxPC-3, CaPAN-2, Mia PaCa-2, PANC-1, and SW1990 cells. Relative FAM126A gene expression was normalized to GAPDH. B Overexpression of FAM126A in BxPC-3 cells, and knockdown FAM126A in PANC-1 cells. Cells were transfected with lentiviruses to generate BxPC-3-vector/LV-FAM126A and PANC-1-NC/sh-FAM126A#1/sh-FAM126A#3 stable cell lines. FAM126A mRNA (upper panel) and protein (lower panel) amounts were detected as described in (A). C Cell proliferation assessed with CCK-8 in presence or absence of FAM126A silencing in BxPC-3 (upper panel) or PANC-1 (lower panel) cells. D, E FAM126A’s effects in clonogenic and EdU assays in PC cells. Representative images and quantitation are depicted in left and right panels, respectively. F Cell cycle analysis after FAM126A silencing and overexpression in BxPC-3 and PANC-1 cells. Representative images and quantitation are depicted in left and right panels, respectively. G The wound healing assay was carried out to evaluate migration in FAM126A-treated BxPC-3 and PANC-1 cells following scratching for 48 h. H The transwell assay evaluated cell migration in FAM126A-treated BxPC-3 and PANC-1 cells. *P < 0.05 **P < 0.01, ***P < 0.001.

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