Fig. 3: FAM126A induces PC progression.

A The amounts of cell cycle-related proteins in FAM126A-silenced or overexpressing cells were examined by immunoblot, with GAPDH for normalization. B Phosphorylated PI3K and Akt amounts in PANC‑1 and BxPC‑3 cells were examined by immunoblot. C In GEPIA2, FAM126A was positively correlated with the EMT-associated transcription factor Snail (R = 0.51, P < 0.001) and Vimentin (R = 0.6, P < 0.001) in PC tissue samples. D Mesenchymal biomarkers, i.e., N-cadherin, Vimentin, and Snail, were downregulated significantly in FAM126A knockdown cells, while the epithelial marker E-cadherin was upregulated. FAM126A overexpression downregulated E-cadherin, while increasing N-cadherin, Vimentin, and Snail amounts. The internal control was GAPDH. *P < 0.05 **P < 0.01, ***P < 0.001.