Fig. 5: Stat1 is a key factor in regulating senescence of osteoblast. | Cell Death Discovery

Fig. 5: Stat1 is a key factor in regulating senescence of osteoblast.

From: BMP9 reduces age-related bone loss in mice by inhibiting osteoblast senescence through Smad1-Stat1-P21 axis

Fig. 5

A GO analysis of differentially expressed genes between replicative senescent MC3T3-E1 cells treated with vehicle or BMP9 for 3 days. B Top 10 downregulated pathways in KEGG analysis. C Overlap analysis of upregulated genes between normal control with replicative senescent MC3T3-E1 cells and downregulated genes between senescent MC3T3-E1 cells treated with vehicle or BMP9. D Heatmap of Stat1 and its downstream target genes in passage 7 and passage 17 of MC3T3-E1 treated with vehicle or BMP9 for 3 days. E qPCR analysis of mRNA level of Stat1 in cells of passage 7 and passage 17 with vehicle or BMP9 treatment for 3 days. F qPCR analysis of mRNA level of Stat1 in control and H2O2-stimulating MC3T3-E1 cells treated with vehicle or BMP9 for 3 days. G qPCR analysis of mRNA levels of Stat1 in 6-month-old and 20-month-old mice treated with AAV-CON or AAV-BMP9 (n = 6). Data presented as mean ± SD. n = 3 biological replicates for in vitro experiment. One-way ANOVA was used for comparison among multiple groups. *P < 0.05; **P < 0.01; ***P < 0.001. Ns, no significance.

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