Fig. 6: The suppression of Stat1 is crucial for the anti-aging effect of BMP9 in replicative senescence of osteoblast. | Cell Death Discovery

Fig. 6: The suppression of Stat1 is crucial for the anti-aging effect of BMP9 in replicative senescence of osteoblast.

From: BMP9 reduces age-related bone loss in mice by inhibiting osteoblast senescence through Smad1-Stat1-P21 axis

Fig. 6

Passage 7 of MC3T3-E1 cells were set as control group, passage 17 of MC3T3-E1 cells were treated with vehicle or BMP9 or BMP9 + 2-NP for 3 days, respectively. A, B qPCR analysis of Stat1 and P21 expression. C Western blot analysis of protein level of Stat1. D Immunofluorescence analysis of protein level of P21. (Scale bar, 50 μm). E, F qPCR analysis of mRNA levels of SASPs. G, H β-galactosidase staining was performed (G) and number of β-gal (+) cells was counted for each group (H) (Scale bar, 100 μm). I Immunofluorescence analysis of γ-H2AX expression (Scale bar, 50 μm). J Alp staining of MC3T3-E1 cells suffered to osteogenic differentiation induction for 7 days. K, L qPCR analysis of mRNA levels of osteoblastic differentiation markers. Data presented as mean ± SD. n = 3 biological replicates. One-way ANOVA was used for comparison among multiple groups. *P < 0.05; **P < 0.01; ***P < 0.001. Ns, no significance.

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