Fig. 3: CircEIF3H interacts with IGF2BP2 and HuR as a scaffold. | Cell Death Discovery

Fig. 3: CircEIF3H interacts with IGF2BP2 and HuR as a scaffold.

From: CircEIF3H-IGF2BP2-HuR scaffold complex promotes TNBC progression via stabilizing HSPD1/RBM8A/G3BP1 mRNA

Fig. 3

A IGF2BP2 protein-specific peptides TVNELQNLTSAEVIVPR and IAPAEGPDVSER were identified by mass spectrometry. B HuR protein-specific peptides VLVDQTTGLSR and NVALLSQLYHSPAR were identified by mass spectrometry. C Western blot of the proteins from circEIF3H and antisense circEIF3H pull-down assay. D Co-IP followed by western blot confirmed the interaction between IGF2BP2 and HuR. E RIP assays in 293 T cells showed the association of circEIF3H with Flag-tagged IGF2BP2 and HA-tagged HuR. IgG served as a negative control. F RIP assays in MDA-MB-231 cells show the association of circEIF3H with Flag-tagged IGF2BP2 and HA-tagged HuR. G Western blot of IGF2BP2 and HuR in samples pulled down by full-length (FL) or truncated circEIF3H (Δ1: 1–80, Δ2: 81–160, Δ3: 161–240, Δ4: 241–320, Δ5: 321–418). The quantitative data were presented as mean ± SD. Statistical significance was determined by two-sided Student’s t-test, *P < 0.05, **P < 0.01, and ***P < 0.001. Cell experiments were repeated three times.

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