Fig. 1: Effects of SFN on BDNF transcription in vitro.
From: Regulation of BDNF transcription by Nrf2 and MeCP2 ameliorates MPTP-induced neurotoxicity
A Chemical structure of SFN. B SFN enhanced Bdnf exon I expression via Nrf2 activation. Following treatment with SFN or/and Nrf2 siRNA, SH-SY5Y cells were collected and analyzed by real-time PCR for Bdnf exon I expression. Data are shown as mean ± SEM (n = 4). *P < 0.05; ***P < 0.001 (one-way ANOVA: F2,11 = 76.90, P < 0.001). C Nrf2 knocking down abolished the activation of Bdnf exon I promoter in HEK293T cells. HEK293T cells were treated with SFN or/and siRNA, followed by luciferase reporter analysis. Data are shown as mean ± SEM (n = 4). ***P < 0.001 (one-way ANOVA: F2,11 = 29.579, P < 0.001). D Mutations of the Nrf2-binding motif in Bdnf exon I promoter blocked BDNF transcription. HEK293T cells were treated with SFN in the presence of wild-type or mutated Bdnf exon I promoter followed by luciferase reporter analysis. Data are shown as mean ± SEM (n = 4). ***P < 0.001 (one-way ANOVA: F2,11 = 198.666, P < 0.001). E Effect of SFN on Nrf2, MeCP2, and BDNF expression in SH-SY5Y cells. Cells were treated with SFN (1 µM) for 24 h, followed by western blotting analysis with Nrf2, MeCP2, and BDNF antibody. β-actin levels were used as a loading control. Data are shown as mean ± SEM (n = 5). *P < 0.05 (Student t test: T = −2.365, P = 0.046 for Nrf2, T = 4.082, P = 0.004 for MeCP2, and T = −2.745, P = 0.025 for BDNF).
