Fig. 2: Effects of SFN on MPP+-induced downregulation of BDNF in SH-SY5Y cells.
From: Regulation of BDNF transcription by Nrf2 and MeCP2 ameliorates MPTP-induced neurotoxicity
A Effects of SFN on the expressions of Nrf2, MeCP2, and BDNF in MPP+-treated SH-SY5Y cells. Cells were treated with SFN (1 μM) and/or MPP+ (1 mM) 24 h, followed by western blotting assay. Data are shown as mean ± SEM (n = 4). *P < 0.05, **P < 0.01 (one-way ANOVA: Nrf2: F2,11 = 5.266, P = 0.031, MeCP2: F2,11 = 10.110, P = 0.005, BDNF: F2,11 = 8.191, P = 0.009). B, C The immunofluorescence staining for Nrf2 and MeCP2 in SH-SY5Y cells treated with SFN (1 μM) and/or MPP+ (1 mM) treated for 24 h. Scale bar = 50 μm. There were statistical differences among the three groups (one-way ANOVA, Nrf2: F2,11 = 17.897, P = 0.001, MeCP2: F2,11 = 19.030, P = 0.001).
