Fig. 6: EGFR knockdown reversed the effects of CB1 silencing on tumor cell growth and M2 macrophage polarization. | Cell Death Discovery

Fig. 6: EGFR knockdown reversed the effects of CB1 silencing on tumor cell growth and M2 macrophage polarization.

From: Cannabinoid Receptor-1 suppresses M2 macrophage polarization in colorectal cancer by downregulating EGFR

Fig. 6

Colorectal cells were transfected with plasmids bearing shRNA against EGFR and simultaneously treated with AM251. Culture medium for these colorectal cells were taken to incubate PMA-induced THP-1 cells. A QPCR analysis showed that sh-EGFR effectively suppressed the expression of EGFR. B, C Colony formation test demonstrated that EGFR knockdown reversed the enhancement in colony formation induced by AM251. D–I QPCR results demonstrated that EGFR knockdown reversed the CB1 inhibition caused decrease in the expression of IL-6 and TNF-α, and increase in IL-10, CCL22, Arg-1 and CD206. *P < 0.05; **P < 0.01; ***P < 0.001.

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