Fig. 4: The effect of CHREBP on GC cell proliferation in vitro and in vivo.
From: CHREBP suppresses gastric cancer progression via the cyclin D1-Rb-E2F1 pathway
A Flow cytometry of AGS and SGC-7901 cells. B The rate of apoptosis of transfected cells was determined by flow cytometry (Q2 + Q3). C Images of tumors derived from different group transfected with empty vector and p-CHREBP of nude mice. D Tumors were assessed by their volume and average weight. E, Relative CHREBP and cyclin D1 expression were detected in subcutaneous tumor samples by qRT–PCR. F Relative Rb, cyclin D1, p-Rb (ser-780), CHREBP, and E2F1 expression were measured in subcutaneous tumor samples by WB. G Protein levels of Rb, cyclin D1, p-Rb (ser-780), CHREBP, E2F1, and Ki-67 in tumor samples were determined by IHC (scale bar: 50 μm). Data are shown as the mean ± SD in triplicate.
