Fig. 7: CHREBP regulates GC growth via the cyclin D1-Rb-E2F1 pathway.

A Relative expression levels of CHREBP, cyclin D1, Rb, E2F1, and Ki-67 were detected in GC cells transfected with scramble, Sh-CHREBP, and an empty vector, p-CHREBP, as determined by qRT–PCR. B WB was used to detect the levels of Rb, cyclin D1, tubulin and phosphorylated Rb (P-Rb), CHREBP, E2F1, and GAPDH in GC cells transfected with scramble, Sh-CHREBP and an empty vector, p-CHREBP. C Concision model of CHREBP in regulating GC progression. The downregulated expression level of CHREBP specifically bound to cyclin D1 promoter, which increased the ability of cyclin D1 to inactivate Rb, leading to the release of E2F1, which could regulate the levels of genes involved in the cell cycle.