Fig. 7: USP7 regulates myocardial ischemia/reperfusion by deubiquitinating and stabilizing Keap1.
From: Ubiquitin-specific protease 7 regulates myocardial ischemia/reperfusion injury by stabilizing Keap1
a After 30 minutes of ischemia and 2 h of reperfusion, the mouse heart tissue was taken for Co-IP. USP7’s interaction with Keap1 was investigated using Western blot. b Co-IP was performed with Keap1, then immunoblotted with antibodies against ubiquitin (Ub) and Keap1. b sh-USP7 was injected into the myocardium of mice, and the myocardial ischemia/reperfusion model was established 7 days later. Immunoblot was used to determine the amounts of ubiquitin (Ub) and Keap1 proteins. c Co-IP was performed with Keap1 and IgG, then immunoblotted with antibodies against Ub and Keap1. Quantitative analysis statistics are shown. d A proposed mechanism for USP7 to regulate myocardial ischemia/reperfusion via stabilizing the Keap1 level. (Created with BioRender.com).
