Fig. 3: Effect of hypoxic preconditioning on proliferating neuroblasts in dentate gyrus of HI mice.
From: Preconditioning in hypoxic-ischemic neonate mice triggers Na+-Ca2+ exchanger-dependent neurogenesis
A Representative confocal images of dentate gyrus of control, HI, and PC + HI groups. Single staining of BrdU (A, B, C), PSA-NCAM (D, E, F), Hoechst (G, H, I), and merge (J, K, L). Scale bar 75 μm. Rectangular box indicates area of high magnification ×500 zoom. B Cell-counting analysis of neuroblasts expressed as the total number of PSA-NCAM+ cells in DG of P11 mice. C Cell-counting analysis of proliferating neuroblasts expressed as the total number of BrdU+/PSA-NCAM+ cells in DG of P11 mice. B, C Data are expressed as mean ± SD (n = 6). *P < 0.05 versus control mice. #P < 0.05 versus mice group subjected to only HI. P-values were obtained by using one-way ANOVA with Newman–Keuls correction for multiple comparisons. D Representative confocal images of DG of control, HI, and PC + HI groups. Single staining of NeuroD1 (A, B, C), Hoechst (D, E, F), and merge (G, H, I). Scale bar 75 μm. Rectangular box indicates area of high magnification ×500 zoom. E Cell-counting analysis of immature neurons expressed as total number of NeuroD1+cells in DG of P11 mice. Data are expressed as mean ± SD (n = 3). *P < 0.05 versus control mice. #P < 0.05 versus mice group subjected to only HI. P-values were obtained by using one-way ANOVA with Newman–Keuls correction for multiple comparisons.
