Fig. 5: Effect of NCX1 silencing on preconditioning-induced neuroprotection.
From: Preconditioning in hypoxic-ischemic neonate mice triggers Na+-Ca2+ exchanger-dependent neurogenesis
A The panel shows the experimental design. The mice were divided into two groups: the first group was subjected to i.c.v. administration of siControl and HPC at P7; in the second the mice were subjected to i.c.v. administration of siNCX1 and HPC at P7, subsequently, all animals were subjected to HI insult at P10. All animals were sacrificed 24 h after injury. B Representative confocal images of hippocampus of two groups. Single staining of NCX1 (A, B); Hoechst (C, D), and merge (E, F, G, H, I, J) in hippocampus of two groups. Scale bar: 200 μm (E, F), 75 μm (G, H), 25 μm (I, J). Rectangular box indicates area of higher magnification. MOL, molecular layer; GCL, granular cell layer. C On top of the figure are included representative rostral-caudal brain sections stained with cresyl violet of preconditioned hypoxic-ischemic (siControl) and preconditioned hypoxic-ischemic (siNCX1) mice. Brain damage induced in mice was evaluated as % of ischemic damage at postnatal day eleven. Data are expressed as mean ± SD (n = 3–4). *P < 0.05 versus preconditioned hypoxic-ischemic (siControl) mice. P-values were obtained by using Unpaired t-test.
