Fig. 1: Genome-Scale CRISPR/Cas9 Screen identified miR-15a and miR-20b involved in sorafenib response. | Cell Death Discovery

Fig. 1: Genome-Scale CRISPR/Cas9 Screen identified miR-15a and miR-20b involved in sorafenib response.

From: miR-15a and miR-20b sensitize hepatocellular carcinoma cells to sorafenib through repressing CDC37L1 and consequent PPIA downregulation

Fig. 1

A Schematic of screening for sorafenib resistance-associated genes in HCC-LM3 cells. B Heat map displayed the rank of sgRNA abundance according to enrichment levels in sorafenib-resistant HCC-LM3 cells. C CCK8 assays were performed in HCC-LM3 cells with silencing of targeting gene as indicated, respectively (one-way ANOVA). D The expression analysis of miR-15a and miR-20b by qRT-PCR (unpaired t test, two-tailed). E The ablation of miR-15a and miR-20b improved cell viability under sorafenib (7.5 μM) treatment (one-way ANOVA). *p < 0.05, **p < 0.01.

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