Fig. 3: CDC37L1 is a common target of miR-15a and miR-20b. | Cell Death Discovery

Fig. 3: CDC37L1 is a common target of miR-15a and miR-20b.

From: miR-15a and miR-20b sensitize hepatocellular carcinoma cells to sorafenib through repressing CDC37L1 and consequent PPIA downregulation

Fig. 3

A Four computational algorithms predicted the potential targets of miR-15a or miR-20b. B Original and mutated sequences of miR-15a or miR-20b-binding elements on the 3′UTR of CDC37L1 mRNA are shown. C Relative luciferase activity in miR-15a or miR-20b overexpressed HCC-LM3 cells with the CDC37L1 3′UTR WT or MUT, respectively (one-way ANOVA). D qRT-PCR was employed to measure CDC37L1 mRNA levels in HCC cells transfected with miR-15a or 20b mimics (one-way ANOVA). E CDC37L1 protein levels were examined in HCC cells after transfection of miR-15a or 20b mimics or inhibitors by western blot analysis. The data are presented as the mean ± SD from three independent experiments, *p < 0.05, **p < 0.01.

Back to article page