Fig. 5: Knockdown of EGFR reversed the inhibitory effect of rasfonin on autophagy. | Cell Death Discovery

Fig. 5: Knockdown of EGFR reversed the inhibitory effect of rasfonin on autophagy.

From: Grb2 interacts with necrosome components and is involved in rasfonin-induced necroptosis

Fig. 5: Knockdown of EGFR reversed the inhibitory effect of rasfonin on autophagy.The alternative text for this image may have been generated using AI.

A ACHN cells were treated with rasfonin (12 μM) with or without gefitinib (5 μM) for 4 h. B After transfection with the control (Mock) or EGFR siRNA (siEGFR) for 48 h, ACHN cells were treated with rasfonin (12 μM) for 4 h. Cell lysates in (A, B) were analyzed by immunoblotting with the indicated antibodies. C The efficiency of siRNA interference was determined with sample in (B). D Schematic representation of the WT EGFR and K721A EGFR. E, F HEK293T cells were transfected transiently with the WT EGFR or K721A EGFR plasmids for 36 h, and treated with rasfonin (12 μM) for 4 h. Cell lysates were analyzed by immunoblotting with the indicated antibody. Actin was used as a loading control. Similar experiments were repeated at least three times.

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