Fig. 6: mmu_circ_0000021 suppression maintained the microvascular barrier following I/R.

A, C Endothelial barrier integrity was assessed using double immunofluorescence labeling of VE-cadherin and CD31. The discontinuity in VE-cadherin expression was ameliorated by knockdown of mmu_circ_0000021. A, D Immunohistochemistry was used to determine microvascular permeability and plasma albumin levels. Absorbed plasma albumin from the microvessels during I/R was reduced by inhibiting mmu_circ_0000021. B, E Microvessel endothelial cell junction were visualized using TEM. Cellular contact and electron dense region are shown in the lower panels, which are from larger images shown in the top panels (cortical protein complex). *P < 0.05, **P < 0.01. vs. indicated group; n = 8/group.