Fig. 6: RBM47 upregulated UPF1 by binding its 3’UTR.
From: RBM47 inhibits hepatocellular carcinoma progression by targeting UPF1 as a DNA/RNA regulator
A Online screenshot of binding site of RBM47 in UPF1 mRNA at starBase 3.0. B RNA decay assay was performed by ActD treatment in Huh7 cells after RBM47 knockdown (left panel) and overexpression in HCCLM3 cells (right panel). Error bars are SD (n = 3). C The direct binding of RBM47 and UPF1 mRNA was detected by RIP-PCR in HCCLM3 cells. D The preferred sequence of the UPF1 3’UTR was identified by motif analysis according to known gene matching. E The effect of RBM47 on the UPF1 3’UTR was tested by a luciferase reporter assay with a 3’UTR mutation vector. Error bars are SD (n = 3). F The direct binding locus was determined by the RNA pulldown assay with a mutant 36 nt probe with predicted binding sites. *P < 0.05, ***P < 0.001.
