Fig. 5: PHF1 overexpression restored the elevated tumor progression induced by FTO inhibition. | Cell Death Discovery

Fig. 5: PHF1 overexpression restored the elevated tumor progression induced by FTO inhibition.

From: Down-regulated m6A reader FTO destabilizes PHF1 that triggers enhanced stemness capacity and tumor progression in lung adenocarcinoma

Fig. 5

A CCK-8 assays showing the in vitro cell growth capacity of FTO-KD cells transfected with vector or PHF1 in three LUAD cell lines (A549, H1299 and PC-9) (N = 5 per group). B Colony formation assay was conducted in FTO-KD A549 cells transfected with vector or PHF1. C Migration assay was conducted in FTO-KD A549 cells transfected with vector or PHF1. D Invasive assay was carried out in FTO-KD A549 cells transfected with vector or PHF1. E Wound-Healing assay was conducted in FTO-KD A549 cells transfected with vector or PHF1. F Quantification of tumor volumes in the H1299-derived tumor model incoporating three groups: shCtrl+vector, shFTO+vector and shFTO+PHF1. G Quantification of tumor weight in the H1299-derived tumor model incoporating the above three groups. Bar = Mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

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