Fig. 2: Silencing of KDM6B inhibits the migration of BMSCs promoted by TGF-β1. | Cell Death Discovery

Fig. 2: Silencing of KDM6B inhibits the migration of BMSCs promoted by TGF-β1.

From: TGF-β1-induced bone marrow mesenchymal stem cells (BMSCs) migration via histone demethylase KDM6B mediated inhibition of methylation marker H3K27me3

Fig. 2

A–C The siRNA-KDM6B inhibited the increased mRNA and protein expression level of migration-related genes promoted by TGF-β1. D, E The IF showed the CXCR4 expression in presence of TGF-β1 upon siRNA-KDM6B. F, G The siRNA-KDM6B significantly inhibited the actin cortical protrusions formation promoted by TGF-β1. H, I The scratch test showed that siRNA-KDM6B decreased the migration area of BMSCs, and also inhibited the migration area promoted by TGF-β1. J, K Transwell test verified that siRNA-KDM6B inhibited the migrated MSCs number promoted by TGF-β1. (*P < 0.05, **P < 0.01, ***P < 0.001 in comparison with the control group; ##P < 0.01, ###P < 0.001 in comparison with TGF-β1 group. All experiments were repeated three times independently. All error bars ± standard deviation.).

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