Fig. 4: TGF-β1 regulated the gene expression by removing H3K27me3 in the promoter region through KDM6B. | Cell Death Discovery

Fig. 4: TGF-β1 regulated the gene expression by removing H3K27me3 in the promoter region through KDM6B.

From: TGF-β1-induced bone marrow mesenchymal stem cells (BMSCs) migration via histone demethylase KDM6B mediated inhibition of methylation marker H3K27me3

Fig. 4

A–D The results of western blotting showed that the protein expression of H3K27me3 was downregulated by TGF-β1, but this inhibitory effect can be partially reversed by silencing of KDM6B. E, F The levels of H3K27me3 in the promoter regions of N cadherin and FN1 were removed by TGF-β1, IgG and 8 kb downstream of the transcription start site were used as negative control. G, H The siRNA-KDM6B could reverse the decreased levels of H3K27me3 at the promoter regions of N cadherin, and FN1 caused by TGF-β1. (**P < 0.01, ***P < 0.001 in comparison with the control group, ###P < 0.001 in comparison with TGF-β1 group. All experiments were repeated three times independently. All error bars ± standard deviation.).

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