Fig. 2: Upregulation of circPOSTN improves GBM cell proliferation, migration, and tumor angiogenesis in vitro. | Cell Death Discovery

Fig. 2: Upregulation of circPOSTN improves GBM cell proliferation, migration, and tumor angiogenesis in vitro.

From: Circular RNA circPOSTN promotes neovascularization by regulating miR-219a-2-3p/STC1 axis and stimulating the secretion of VEGFA in glioblastoma

Fig. 2

A Cell proliferation ability of GBM cells with or without circPOSTN overexpressing was determined by EdU staining (green). The Edu-positive cells were recorded and quantified. The nuclei were stained with DAPI (blue). B The clone formation assay was used to assess the clone formation capacity of GBM cells with or without circPOSTN overexpressing. C–E The human umbilical vein endothelial cells (HUVECs) were cultured with conditioned media from GBM cells with or without circPOSTN overexpressing. C The viability of HUVEC was evaluated by MTT assay. D The transwell migration assay was used to evaluate the migration ability of HUVECs. E The tubule formation assay was used to assess the angiogenesis ability of GBM cells. The tubule formation ability of HUVEC was analyzed by measuring the branch points and tubule number. Data are presented as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

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