Fig. 4: MiR-514b-3p represses the expression of DUSP4 mRNA. | Cell Death Discovery

Fig. 4: MiR-514b-3p represses the expression of DUSP4 mRNA.

From: Silencing circFTO inhibits malignant phenotype through modulating DUSP4 expression in clear cell renal cell carcinoma

Fig. 4

A. Prediction of the potential target mRNAs of miR-514b-3p. B Boxplot of DUSP4 expression in tumor and control in TCGA KIRC cohort. C Relative expression of 6 genes predicted to be target of miR-514b-3p in 786-O cells with/without knockdown of cricFTO. D Correlation of DUSP4 and circFTO expression in our clinical cohort. E The designs of dual-luciferase reporter vector of wild-type (WT) and mutated (Mut) DUSP4 sequences with binding site to miR-514b-3p. F Relative luc activity after transfection with miR-514b-3p mimic/negative control or with the DUSP4 WT/Mut in HEK293T cells. G DUSP4 level after transfected miR-514b-3p mimics/inhibitors and their control into 786-O and A498 cells detected by Western Blot. H Gene set enrichment analysis (GSEA) in TCGA KIRC cohort according to expression level of DUSP4. Median is used as cutoff.

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