Fig. 5: Pachytene-related genes were significantly repressed in CT47-BAC mice.
From: Human-specific gene CT47 blocks PRMT5 degradation to lead to meiosis arrest
A, B Volcano plot representing differentially expressed genes in the RNA-seq of P14 (A) and P10 (B) in the indicated testes. Volcano plots for three biological replicates of each genotype are shown. Blue dots represent 539 downregulated genes of P14 CT47-BAC testes. Red dots represent one upregulated gene of P10 CT47-BAC testes. The P-adj < 0.05 and log2-fold change ≥1 cutoff values. C Gene ontology functional enrichment (GO) of the DEGs arising from P14 CT47-BAC over WT testes according to the −log (p value). D RNA-seq analysis of selected genes related to pachytene expression. The mean values ± SD are shown. Student’s t test: n = 3 for each group, *p < 0.05, ***p < 0.001. E RNA-seq analysis of piRNA-related genes. The mean values ± SD are shown. Student’s t test: n = 3 for each group, *p < 0.05, **p < 0.01, ***p < 0.001.
