Fig. 1: miR-184-3p is downregulated in human pancreatic islets in T2D and protects β-cells from palmitate- and cytokine-induced apoptosis. | Cell Death Discovery

Fig. 1: miR-184-3p is downregulated in human pancreatic islets in T2D and protects β-cells from palmitate- and cytokine-induced apoptosis.

From: Reduced miR-184-3p expression protects pancreatic β-cells from lipotoxic and proinflammatory apoptosis in type 2 diabetes via CRTC1 upregulation

Fig. 1: miR-184-3p is downregulated in human pancreatic islets in T2D and protects β-cells from palmitate- and cytokine-induced apoptosis.

a RT-Real-time PCR analysis of miR-184-3p expression in human T2D pancreatic islets (n = 9; dark grey squares) and in non-diabetic (n = 13; light grey circles) multi-organ donors; data are presented as 2−ΔCt relative expression single point values normalised with RNU44 and RNU48, along with the mean; statistics with Mann–Whitney U test. b Cytofluorimetric evaluation of cleaved Caspase-3 positive (cleaved-CASP-3+) EndoC-βH1 cells each after 48 h palmitate treatment (*p = 0.0008 vs. untreated miR-Ctr inhibitor; #p = 0.0005 vs. Palmitate-treated miR-Ctr inhibitor transfected) and c after 24 h cytokine mix (IL -1β+TNF-α+IFN-γ) treatment (*p = 0.032 vs. untreated miR-Ctr inhibitor). Data are presented as mean ± SD of the percentage (%) of positive cells to total live cells. Statistics were performed using ANOVA analysis with Bonferroni’s multiple comparison test (n = 4 independent experiments).

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