Fig. 2: CRTC1 is upregulated in LCM-captured human β-cells and in pancreatic islets from T2D donors. | Cell Death Discovery

Fig. 2: CRTC1 is upregulated in LCM-captured human β-cells and in pancreatic islets from T2D donors.

From: Reduced miR-184-3p expression protects pancreatic β-cells from lipotoxic and proinflammatory apoptosis in type 2 diabetes via CRTC1 upregulation

Fig. 2: CRTC1 is upregulated in LCM-captured human β-cells and in pancreatic islets from T2D donors.

a Hierarchical clustering heatmap expression analysis of hsa-miR-184-3p predicted target genes (n = 25) detected in LCM β-cells from n = 10 T2D vs. n = 10 non-diabetic donors (data previously published and deposited in GEO dataset GDS3782-GSE20966). Gene expression values are shown as arbitrary unit count (A.U.) values on a colour scale (high expression: red; low expression: blue). b Dot-plot diagram showing the CRTC1 expression values analysed in the dataset reported in a. The values are reported as arbitrary units (A.U.) along with the mean. c Dot plot showing CRTC1 expression in isolated human pancreatic islets from n = 6 T2D compared to n = 7 non-diabetic donors (data previously published and included in GEO dataset GDS3882-GSE25724). Values are reported as A.U. along with the mean. d RT-Real-time PCR validation of CRTC1 expression in human pancreatic islets isolated from n = 6 T2D vs. n = 10 non-diabetic multi-organ donors; data are reported as 2−ΔCt relative expression single point values normalised by β-ACT and GAPDH; statistics were performed using the Mann–Whitney U test (p < 0.05).

Back to article page