Fig. 5: Cpd16 inhibits dexamethasone- and nicotine-induced oxidative injury in osteoblasts. | Cell Death Discovery

Fig. 5: Cpd16 inhibits dexamethasone- and nicotine-induced oxidative injury in osteoblasts.

From: Nrf2 signaling activation by a small molecule activator compound 16 inhibits hydrogen peroxide-induced oxidative injury and death in osteoblasts

Fig. 5: Cpd16 inhibits dexamethasone- and nicotine-induced oxidative injury in osteoblasts.

The primary murine osteoblasts (A–E), human osteoblasts (F–I), or the MC3T3-E1 murine osteoblastic cells (J, K) were pretreated (for 2 h) with Cpd16 (25 μM), followed with or without dexamethasone (DEX, 2 μM) or nicotine (1 μM) treatments, ROS, depolarization of mitochondria, apoptosis, viability, and necrosis were tested by CellROX staining (A, F), JC-1 staining (B), TUNEL-nuclei staining (C, G), CCK-8 (D, H, J), and LDH releasing (E, I, K) assays, respectively. *P < 0.05. Scale bar = 100 μm (A–C).

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