Fig. 3: mTORC2 signals to PKC to promote ATF4 transcription. | Cell Death Discovery

Fig. 3: mTORC2 signals to PKC to promote ATF4 transcription.

From: Elucidation of an mTORC2-PKC-NRF2 pathway that sustains the ATF4 stress response and identification of Sirt5 as a key ATF4 effector

Fig. 3

A Western blot analysis of WCL from MDA-MB-231 and BT549 cells treated with CB-839 (1 μM) ± Torin1 (500 nM) for 24 h. Blots are representative of three (MDA-MB-231) or two (BT549) independent experiments. B Western blot analysis of WCL from MDA-MB-231 and BT549 cells treated with CB-839 (1 μM) ± Ro31-8220 (5 μM) for 24 h. Blots are representative of two independent experiments. C Western blot analysis of WCL and RT-qPCR quantification of ATF4 mRNA in control and PKCα KD MDA-MB-231 cells treated with CB-839 (1 μM) for 24 h. Blots are representative of three independent experiments. qPCR data shown are representative of two independent experiments and expressed as means ± SD for triplicate measurements. D Western blot analysis of WCL and RT-qPCR quantification of ATF4 mRNA in control and PKCα KD MDA-MB-231 cells deprived of glutamine for 24 h. Blots are representative of two independent experiments. qPCR data shown are representative of two independent experiments and expressed as means ± SD for triplicate measurements.

Back to article page