Fig. 2: Isolation of exosomes from CAFs and NFs. | Cell Death Discovery

Fig. 2: Isolation of exosomes from CAFs and NFs.

From: Cancer associated fibroblasts secreted exosomal miR-1290 contributes to prostate cancer cell growth and metastasis via targeting GSK3β

Fig. 2

A, B Identification of exosomes (CAFs-Exo and NFs-Exo) derived from CAFs and NFs by TEM (Scale bar = 100 nm) and NTA analysis. Yellow arrow points at exosomes. C Western blot analysis of TSG101, CD9 and Calnexin expressions in CAFs-Exo and NFs-Exo. D PC3 cells were treated with PKH26-labeled CAFs-Exo and PKH26-labeled NFs-Exo for 24 h, and the red exosome signal was captured by fluorescence microscopy. Scale bar = 20 μm.

Back to article page