Fig. 3: Substantia Nigra dopaminergic cell survival, inflammation, and α-synuclein load.
From: Inflammatory gut as a pathologic and therapeutic target in Parkinson’s disease
A Immunohistochemical staining of the bilateral substantia nigra pars compacta (SNpc) was conducted to elucidate alterations in cell population expression for α-synuclein (red), TH (green), OX6 (green), and TNF-α (green) with DAPI (blue). Immunofluorescent microscopy was used to collect the images of representative samples from each animal group (Scale bar = 50 um). B ANOVA revealed significantly elevated α-synuclein intensity level (****p < 0.0001), significantly lower TH expression (****p < 0.0001), significantly elevated OX6 expression (****p < 0.0001), and significantly elevated TNF-α expression (****p < 0.0001) in Tg vs Wt animals. Additionally, post hoc Bonferroni’s test analysis revealed P and hUCB+P treatment significantly lowered (*p < 0.05) the inflammatory response in Tg animals (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).
