Fig. 5: CAV2 promotes ubiquitin-proteasome-mediated degradation of S100A14. | Cell Death Discovery

Fig. 5: CAV2 promotes ubiquitin-proteasome-mediated degradation of S100A14.

From: CAV2 promotes the invasion and metastasis of head and neck squamous cell carcinomas by regulating S100 proteins

Fig. 5: CAV2 promotes ubiquitin-proteasome-mediated degradation of S100A14.

A S100A14 protein levels were determined by immunoblotting in vector control or shCAV2 SCC15 cells in the presence of 50 µg/mL CHX for the indicated time periods. B S100A14 protein levels were determined by immunoblotting in control and CAV2-overexpressing SCC15 and SCC25 cells in the presence or absence of the proteasome inhibitor MG132 (50 μM). C, D Ubiquitin-S100A14 was detected by immunoprecipitation (IP) using anti-S100A14 with a subsequent immunoblot assay with anti-ubiquitin antibody in control and shCAV2 SCC15 and SCC25 cells. HNSCC cells were transfected with or without an HA-Ub expression plasmid.

Back to article page