Fig. 5: WWP2 degrades PARP1 through polyubiquitination-proteasome way. | Cell Death Discovery

Fig. 5: WWP2 degrades PARP1 through polyubiquitination-proteasome way.

From: The role of E3 ubiquitin ligase WWP2 and the regulation of PARP1 by ubiquitinated degradation in acute lymphoblastic leukemia

Fig. 5

A Coimmunoprecipitation assays were performed to assess endogenous interaction between WWP2 and PARP1 with or without MG132 treatment in Jurkat cells. B Coimmunoprecipitation assays were performed to assess half-exogenous interaction between WWP2 and PARP1 with or without MG132 treatment in Jurkat cells. C HA-WWP2 and HA-ubiquitin (UB) were co-expressed with or without MG132 treatment in Jurkat cells, PARP1 was purified by IP and PARP1 ubiquitination levels were assessed by anti-HA antibodies. D ShWWP2 and HA-UB were co-expressed with or without MG132 treatment in Jurkat cells, PARP1 was purified by IP and PARP1 ubiquitination levels were assessed by anti-HA antibodies.

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