Fig. 3: ANXA2 induces autophagy and lysosomal functions, and is associated with Wnt/β-catenin signaling. | Cell Death Discovery

Fig. 3: ANXA2 induces autophagy and lysosomal functions, and is associated with Wnt/β-catenin signaling.

From: Annexin A2 plays a key role in protecting against cisplatin-induced AKI through β-catenin/TFEB pathway

Fig. 3

A Heatmap shows the FPKM value of genes in different groups. Relevant genes are categorized into different GSEA gene sets. The color ranges from red to blue represents the log2-ranked FPKM value from large to small. Representative photographs show β-catenin expression (B) and its quantitation (C) in 3 groups. Black arrows indicate positive staining. Scale bars, 50 μm. ***P < 0.001 versus Ctrl (n = 5); ###P < 0.001 versus cisplatin alone (n = 5). Representative western blotting (D) and quantitative data (E) show ANXA2 induces β-catenin activation. Numbers (1–3) indicate individual animals in each group. ***P < 0.001 versus Ctrl (n = 5); ###P < 0.001 versus cisplatin alone. Representative western blotting (F) and quantitative data (G–I) show the expression of Lamp1 (G), Lamp2 (H) and TFEB (I) in three groups. Numbers (1–3) indicate individual animals in each group. **P < 0.01, ***P < 0.001 versus Ctrl (n = 5); ###P < 0.001 versus cisplatin alone (n = 5). J Representative photographs show TFEB (the top panel) and Lamp1 (the bottom panel) expression in 3 groups. Arrows indicate positive staining. Scale bars, 50 μm or 25 μm. K, L Quantification of TFEB and Lamp1 staining are shown. **P < 0.01, ***P < 0.001 versus Ctrl (n = 5); ###P < 0.001 versus cisplatin alone (n = 5). M qPCR results show mRNA levels of TFEB, Lamp1, Lamp2, PSAP, TPP1 and Atp6ap1 in different groups. *P < 0.05, **P < 0.01 versus Ctrl (n = 5); #P < 0.05, ##P < 0.01, ###P < 0.001 versus cisplatin alone (n = 5). Abbreviations: ANXA2 annexin A2, Ctrl control, FPKM fragments per kilobase of transcript per million, GSEA Gene Set Enrichment Analysis, pcDNA3 empty vector.

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