Fig. 8: ANXA2 promotes lysosomal functions through β-catenin/TCF4 signaling. | Cell Death Discovery

Fig. 8: ANXA2 promotes lysosomal functions through β-catenin/TCF4 signaling.

From: Annexin A2 plays a key role in protecting against cisplatin-induced AKI through β-catenin/TFEB pathway

Fig. 8: ANXA2 promotes lysosomal functions through β-catenin/TCF4 signaling.The alternative text for this image may have been generated using AI.

A Representative photographs show the immunofluorescence staining of TFEB (B) and its quantification. White arrows indicate nuclear staining of TFEB. Scale bar, 25 μm. *P < 0.05 versus pcDNA3 (n = 3). C qPCR shows the mRNA levels of TFEB. **P < 0.001 versus pcDNA3 (n = 3). D qPCR results show the mRNA levels of lysosome-related genes. HK-2 cells were transfected with ANXA2 expression plasmid for 24 h. *P < 0.05, **P < 0.01, ***P < 0.001 versus pcDNA3 (n = 3). E, F Representative photographs and quantification show lysosome staining in different groups. Scale bar, 50 μm. HK-2 cells were pretreated with recombinant ANXA2 protein (2 μg/mL) or transfected with siRNA to ANXA2 or negative control (NC) for 24 h, and then treated with cisplatin (25 μg/mL) or medium alone for another 24 h. *P < 0.05, cisplatin (n = 3) versus NC alone (n = 3); #P < 0.05, ANXA2 (n = 3) versus NC alone (n = 3); +P < 0.05, cisplatin+ANXA2 (n = 3) versus ANXA2 + NC (n = 3); φP < 0.05, cisplatin+ANXA2 (n = 3) versus cisplatin+NC (n = 3); ▵P < 0.05, cisplatin+si-ANXA2 (n = 3) versus cisplatin+NC (n = 3). Representative western blotting (G) and quantitative data show the expression of TFEB (H) in different groups. *P < 0.05 versus Ctrl (n = 3); ##P < 0.01 versus Cisplatin alone (n = 3). HK-2 cells were transfected with si-ANXA2 for 24 h, and then treated with cisplatin (25 μg/mL) for 24 h. Abbreviations: TFEB transcription factor EB, Lamp1 lysosomal associated membrane protein 1, TPP1 tripeptidyl peptidase 1.

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