Table 2 List of plasmids.
Lentiviral Vector | psPAX2 | Addgene |
pMD2.G | Addgene | |
pLKO.1 shNontarget | in this study | |
pLKO.1 shLATS1 | in this study | |
pLKO.1 shLATS2 | in this study | |
CRISPR LATS1 gRNA | in this study | |
CRISPR LATS2 gRNA | in this study | |
pLKO-CMV-EGFP-TAZ WT | A Matsushita et al. | |
pLKO-CMV-EGFP-TAZ S89A | A Matsushita et al. | |
pLKO-CMV-EGFP-YAP1 WT | in this study | |
pLKO-CMV-EGFP-YAP1 S127A | in this study | |
Expression vector | pCMV-SMG6 WT | in this study |
pCMV-SMG6 D1353A | in this study | |
pCMV-SMG6 R1407A | in this study | |
pCMV-SMG6 F1412A | in this study | |
pCMV-SMG6 W1415A | in this study | |
pBABE-TERT WT | M Yasukawa et al. | |
pBABE-TERT D712A | M Yasukawa et al. | |
pBABE-TERT T249A | M Yasukawa et al. | |
Retroviral Expression system | MSCV-YAP WT-IRES-GFP | T Kakiuchi et al. |
MSCV-YAP S127A-IRES-GFP | T Kakiuchi et al. |
