Fig. 2: USP22 interacts with FASN and inhibits FASN ubiqutination. | Cell Death Discovery

Fig. 2: USP22 interacts with FASN and inhibits FASN ubiqutination.

From: Derepression of the USP22-FASN axis by p53 loss under oxidative stress drives lipogenesis and tumorigenesis

Fig. 2: USP22 interacts with FASN and inhibits FASN ubiqutination.The alternative text for this image may have been generated using AI.

A RKO E6 and HT29 cell lysates were immunoprecipitated using antibodies against USP22 and FASN, and then subjected to immunoblotting. IgG was used as an isotype control. B Immunofluorescence assays were performed in RKO E6 and HT29 cells using antibodies against USP22 and FASN. Scale bar, 20 μM. Insets: high-magnification images. C A series of Flag-tagged FASN deletion mutants were constructed and co-transfected with HA-USP22 into 293T cells. Cell lysates were immunoprecipitated using an anti-Flag antibody and then analyzed by immunoblotting. The domains of FASN protein are indicated (left panel). D 293T cells were transfected with Flag-FASN, Myc-Ubi, and HA-USP22, and then treated with MG132 for 6 h. Cell lysates were immunoprecipitated using an anti-Flag antibody and then analyzed by immunoblotting. E RKO E6 cells were transfected with USP22 siRNA and Myc-Ubi, and then treated with MG132 for 6 h. Cell lysates were immunoprecipitated using an anti-FASN antibody and then analyzed by immunoblotting.

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