Fig. 8: RACK1 downregulation affects D. melanogaster muscle regeneration. | Cell Death Discovery

Fig. 8: RACK1 downregulation affects D. melanogaster muscle regeneration.

From: RACK1 is evolutionary conserved in satellite stem cell activation and adult skeletal muscle regeneration

Fig. 8: RACK1 downregulation affects D. melanogaster muscle regeneration.

A, D Confocal fluorescence imaging of Phalloidin (blue) or TO-PRO (red) in DLM longitudinal sections of Mef2-Gal4/Zfh1-Gal4 (controls) and Mef2>RACK1 IR/Zfh1>RACK1 IR flies after physical damage at increasing times, and B, E time-course measurement of the wound area. Results are expressed as percentage of the wound area of the respective strain measured just after damage (t = 0 h, dotted line). ****P < 0.0001 vs Mef2>RACK1 IR/Zfh1>RACK1 IR. Images and quantitative data are representative of 20 ≤ n ≤ 30 flies. C, F Confocal fluorescence imaging of Jagged1 (green) and RACK1 (red) in DLM longitudinal sections of Mef2-Gal4/Zfh1-Gal4 (controls) and Mef2>RACK1 IR/Zfh1>RACK1 IR flies 5 days after physical damage. Lower panels: mean Jagged1 intensity signals (A.U.: arbitrary units) around the injury. ***P < 0.001 vs the respective control. Images are representative of 10 ≤ n ≤ 15 flies. G Confocal fluorescence imaging of ATF4 (red) and Phalloidin (blue) in DLM longitudinal sections of Zfh1-Gal4 (controls) and Zfh1>RACK1 IR flies 5 days after physical damage. Images are representative of 10 flies. Scale bars: 20 µm.

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